proteasome-glo 3-substrate cell based assay system (Promega)

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Promega proteasome-glo 3-substrate cell based assay system
Proteasome Glo 3 Substrate Cell Based Assay System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteasome-glo+3-substrate+cell-based+assay+system/proteasome+glotm+chymotrypsin+like+cell+based+assay/pmc10564874-186-6-12
Average 90 stars, based on 1 article reviews

proteasome-glo 3-substrate cell based assay system - by Bioz Stars, 2026-10

90/100 stars

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Activity Assay:

Article Title: Insulin Suppresses Ubiquitination via the Deubiquitinating Enzyme Ubiquitin-Specific Protease 14, Independent of Proteasome Activity in H4IIEC3 Hepatocytes.
Article Snippet: Ubiquitin-proteasome dysfunction contributes to obesity-related metabolic disorders, such as diabetes and fatty liver disease.. However, the regulation of ubiquitin-proteasome activity by insulin remains to be elucidated.. Here, we show that prolonged insulin stimulation activates proteasome function even though it reduces the ubiquitinated proteins in H4IIEC3 hepatocytes.

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: Reagent or resource Source Identifier Antibodies Mouse IgG1 anti-Myosin heavy chain type IIA DSHB SC-71; RRID:AB_2147165 Mouse IgM anti-Myosin heavy chain type IIB DSHB BF-F3; RRID:AB_2266724 Rat anti-laminin a-2 (4H8-2) Santa Cruz Sc-59854; RRID:AB_784266 Mouse anti-ubiquitin (P4D1) Santa Cruz Sc-8017; RRID:AB_2762364 Rabbit anti-p62/SQSTM1 Cell Signaling Technologies 5114S; RRID:AB_10624872 Rabbit anti-alpha- tubulin (11H10) Cell Signaling Technologies 2125S; RRID:AB_2619646 Rabbit anti-GAPDH (D16H11) Cell Signaling Technologies 5174S; RRID:AB_10622025 Rat anti-F4/80 BioLegend 123101; RRID:AB_893504 Rat anti-Ly6G (1A8) BioLegend 127601; RRID:AB_1089179 Mouse anti-Cyr61 (A-10) Santa Cruz sc-374129; RRID:AB_10947399 Mouse anti-Clusterin/ApoJ R&D AF2747-SP; RRID:AB_2083314 Rabbit anti-ApoD Novus Bio NBP2-92650; RRID:N/A Rabbit anti-Calsequestrin1 Novus Bio NBP1-88180; RRID:AB_11010381 AlexaFluor488 anti-mouse IgG1 Life Technologies A21121; RRID:AB_2535764 AlexaFluor555 anti-mouse IgM Life Technologies A21426; RRID:AB_1500929 AlexaFluor647 anti-rat Life Technologies A21247; RRID:AB_141778 Anti-mouse IgG, HRP-linked Cell Signaling Technologies 7076S; RRID:AB_330924 Anti-rabbit IgG, HRP-linked Cell Signaling Technologies 7074S; RRID:AB_2099233 Chemicals, peptides, and recombinant proteins AlexaFluor635 Phalloidin Life Technologies A22284 Dexamethasone Sigma-Aldrich D2915 DAPI Roche 10236276001 TRIzol Ambion 15596018 MG132 Sigma-Aldrich M8699 IQ Sybr Green supermix Bio-Rad 170-8885 6-well plates Corning REF3516 96-well PCR plates Biorad HSP9601 Transparent 96-well plates Corning REF3599 High glucose DMEM, with glutamax GIBCO 10566016 Fetal bovine serum GIBCO 10437-028 Penicillin streptomycin GIBCO 15140122 PBS GIBCO 10010023 Blue loading buffer pack Cell Signaling 7722 Precision Plus protein standard Bio-Rad 1610374 4-20% Mini-PROTEAN TGX pre-cast gels Bio-Rad 4561096 Immobilon-P PVDF membrane Millipore IPVH00010 Ponceau S Sigma-Aldrich P7170-1L 16% Paraformaldehyde Electron Microscopy Sciences 15710 Critical commercial assays Pierce BCA protein assay kit Thermo Scientific 23225 Proteasome-Glo 3-substrate cell-based assay system Promega G1180 iScript reverse transcriptase Bio-Rad 1708840 Deposited data (Continued on next page) Cell Reports 37, 109971, November 9, 2021 e1

Article Title: Firefly Luciferase and Rluc8 Exhibit Differential Sensitivity to Oxidative Stress in Apoptotic Cells
Article Snippet: Proteasome activity measurements were obtained using the Proteasome-Glo 3-Substrate Cell-Based Assay system (Promega) according to the manufacturer's instructions.

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Luminescence Assay:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Cell Based Assay:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Recombinant:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

SYBR Green Assay:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Polymerase Chain Reaction:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Membrane:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Electron Microscopy:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Bicinchoninic Acid Protein Assay:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Reverse Transcription:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Muscles:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Gene Expression:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Control:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Mass Spectrometry:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

Software:

Article Title: Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy
Article Snippet: 10 μL of the normalized NP40 muscle extracts were then used for the measurement of proteasome activity with the Proteasome-Glo 3-substrate cell-based assay system (Promega #G1180), as previously done ( ; ).

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proteasome-glo 3-substrate cell based assay system (Promega)

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Related Articles

Activity Assay:

Luminescence Assay:

Cell Based Assay:

Recombinant:

SYBR Green Assay:

Polymerase Chain Reaction:

Membrane:

Electron Microscopy:

Bicinchoninic Acid Protein Assay:

Reverse Transcription:

Muscles:

Gene Expression:

Control:

Mass Spectrometry:

Software:

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